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. 2021 Apr 20;10(4):963. doi: 10.3390/cells10040963

Figure 1.

Figure 1

Schematic illustration of the experimental design of BBB models that were used in this study. “EC_only“: endothelial cells (ECs) are plated on the apical side of a 24-well-plate insert with a pore-size of 0.4 μm. “indirect“: Pericytes (PCs) are plated on the basolateral side of the insert, and in a second step (after 3 h), ECs are plated on the apical side, allowing contact of the two cell types through the membrane. “PC_EC direct“: PCs are plated on the apical side of inserts first, and after 3 h, ECs are plated on top of PCs. “EC_PC direct“: ECs are plated first, and after 3 h, PCs are seeded on top of them.