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. 2021 Apr 23;9:e11094. doi: 10.7717/peerj.11094

Figure 3. Role of cell–cell contact in the Th17 cell response induced by P. gingivalis lipopolysaccharide-activated CD14+ monocytes.

Figure 3

CD14+ monocytes and CD4+ T cells were cultured in transwells for 3 days, and the expression of RORC (A) and IL-17 (B) mRNA in the presence or absence of P. gingivalis lipopolysaccharide was determined. (C) Dot plots show the proportion of Th17 cell differentiation determined using flow cytometry. (D) Frequency of Th17 cells in each group determined by using flow cytometry. Data are presented as mean ± SD of triplicate assays for six independent experiments (n = 6). Transwell (TW), lipopolysaccharide (LPS), retinoid-related orphan receptor C (RORC), no significant difference (ns).