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. Author manuscript; available in PMC: 2021 Dec 3.
Published in final edited form as: Mol Cell. 2020 Nov 25;80(5):903–914.e8. doi: 10.1016/j.molcel.2020.11.014

Figure 2.

Figure 2.

fSHAPE identifies sites bound by proteins. (a) Example replicate averaged fSHAPE reactivity profiles from four cell types across the iron response element in the Ferritin light chain (FTL) transcript. (b) The predicted secondary structure of the FTL iron response element (left), bases colored according to fSHAPE reactivity and numbered by position in FTL transcript NM_000146. Asterisks indicate bases known to hydrogen bond with iron response element binding protein (IRP1), based on the crystal structure (right; PDBID: 3SNP)(Walden et al., 2006) of IRP1 (green) bound to the FTL iron response element (black). See also Tables S2–3.