. 2021 Apr 20;10(4):820. doi: 10.3390/plants10040820

Table 1.

Effects of different NaCl concentrations (0, 500, 1000, 2000, and 3000 mg L⁻¹) on callus and plantlets vegetative growth characteristics of stevia varieties (Sugar High A3 and Spanti) in vitro.

Parameters	NaCl mg L⁻¹ Concentrations	Stevia Varieties		Means
Parameters	NaCl mg L⁻¹ Concentrations	Sugar High A3	Spanti	Means
Survival callus%	Control	62.50 ^a	62.00 ^a	62.25 ^a
	500	53.09 ^b	50.60 ^b,c	51.85 ^b
	1000	52.30 ^b	43.66 ^d	47.98 ^c
	2000	48.05 ^c	36.17 ^e	42.11 ^d
	3000	44.20 ^d	32.65 ^f	38.43 ^e
	Means	52.13 ^a	45.01 ^b
Regeneration%	Control	42.11 ^a	34.93 ^b,c	38.52 ^a
	500	35.83 ^a,b	30.16 ^c	32.99 ^b
	1000	36.03 ^a,b	23.12 ^d,e	29.57 ^c
	2000	35.61 ^b,c	20.14 ^e	27.88 ^c,d
	3000	29.81 ^c	18.09 ^e	23.95 ^d
	Means	35.90 ^a	25.30 ^b
Plantlet length (cm)	Control	5.15 ^b	4.86 ^b	5.01 ^a,b
	500	5.66 ^a	4.44 ^b	5.05 ^a
	1000	4.73 ^b	4.23 ^b	4.48 ^a,b
	2000	4.26 ^b	3.74 ^b	4.00 ^b
	3000	4.02 ^b	3.13 ^b	3.58 ^b
	Means	4.76 ^a	4.08 ^b
No. of leaf plantlets⁻¹	Control	9.61 ^a	8.76 ^ab	9.19 ^a
	500	8.83 ^a,b	7.51 ^a,c	8.17 ^a,b
	1000	7.56 ^a,b,c	7.02 ^b,c,d	7.29 ^b
	2000	7.15 ^b,c,d	5.53 ^d	6.34 ^c
	3000	6.11 ^c,d	5.31 ^d	5.71 ^c
	Means	7.85 ^a	6.82 ^b
No. of multiple shoots	Control	15.60 ^a	12.50 ^b,c,d	14.05 ^a
	500	14.42 ^a,b	10.60 ^d,e,f	12.51 ^b
	1000	13.27 ^a,b	8.36 ^e,f,g	10.82 ^b,c
	2000	12.87 ^b,c	7.68 ^f,g	10.28 ^c
	3000	10.23 ^c,d,e	6.68 ^g	8.46 ^d
	Means	13.22 ^a	9.16 ^b
Root length (cm)	Control	7.55 ^a	6.57 ^c,d	7.06 ^a
	500	7.22 ^a,b	6.23 ^c,d	6.73 ^a
	1000	6.71 ^b,c	6.23 ^d,e	6.47 ^b
	2000	6.17 ^c,d,e	5.21 ^f	5.49 ^c
	3000	6.06 ^e	5.85 ^f	5.96 ^c
	Means	6.66 ^a	6.02 ^b
No. of root plantlets⁻¹	Control	7.26 ^a	6.27 ^a,b	6.77 ^a
	500	7.31 ^a	5.05 ^c	6.18 ^a,b
	1000	6.38 ^a,b	5.11 ^c	5.75 ^b
	2000	5.26 ^b,c	3.36 ^d	4.31 ^c
	3000	5.01 ^c	3.76 ^d	4.39 ^c
	Means	6.24 ^a	4.96 ^b

Means followed by the same letters in each mean column, mean row, or interaction are not significantly different at the 5% level.