Skip to main content
. Author manuscript; available in PMC: 2021 Apr 26.
Published in final edited form as: Small. 2020 Apr 27;16(21):e2000963. doi: 10.1002/smll.202000963

Figure 3. Defective Nrf2-dependent ROS signaling in inflamed SAEC deregulates nano-adaptive response.

Figure 3.

(A) Representative immunofluorescence images of ZnO, CuO, and Nano-CCP conditioned (24h) normal and inflamed SAEC counterstained for Nrf2 (green). Cell nucleus (blue) was labeled using Hoechst 33342 dye. Scale bar = 50 μm. (B) Normalized expression of cell nucleus-bound Nrf-2 in normal and inflamed SAEC. n=20 per group. Time-course measurement of intracellular ROS level in (C) normal and (D) inflamed SAEC exposed to ZnO, CuO and Nano-CCP over 24h of NM treatment time. Mean (E) total antioxidant capacity and (F) proteolytic activity in normal and inflamed SAEC 24h post NM conditioning. Experiments were conducted in triplicates. Data are presented as mean ± SD. * and ** denote statistical significance between experimental groups. *: p < 0.05, and **: p < 0.01.