Recovery of male reproductive fitness following exposure to single- or double-heat stress conditions (a), or immature versus mature adult male life stages. (a) Male reproductive fitness following exposure to 30°C control (white, n = 63), single 42°C heat stress conditions (red, n = 63) and double 42°C heat stress conditions (orange, n = 28). (b) Male reproductive fitness following exposure to 30°C control (white circles, n = 19), a 42°C period of heat stress during the immature adult stage (orange squares, n = 10), and 42°C heat stress exposure to the mature adult life stage (red triangles, n = 16). Mature males were not assayed in the day 1 time-point as, being from the same cohort, they were then undergoing heat stress treatment. Within time-point significance threshold: *** = p < 0.001.