TABLE 3.
Studies of embryo development after silencing of ADAMTS1.
si-Ctrl (n = 48) | si-ADAMTS1 (n = 48) | p-value | |
Development time to morula stage (h) | 68.21 ± 2.66 | 77.05 ± 3.09 | 0.037 |
Good-quality embryo rate (%) | 76.3 | 65.8 | 0.312 |
Morula formation rate (%) | 47.4 | 47.4 | 1.000 |
Blastocyst formation rate (%) | 28.9 | 31.6 | 0.803 |
Good-quality blastocyst rate (%) | 27.3 | 25.0 | 0.901 |
The development time to morula stage is calculated from the time of siRNA injection. Good-quality embryo rate (%) = (number of D3 good-quality embryos/number of cleaved embryos) × 100; Morula formation rate (%) = (number of morulae/number of embryos cultured) × 100; Blastocyst formation rate (%) = (number of blastocysts/number of embryos cultured) × 100; Good-quality blastocyst rate (%) = (number of good-quality blastocysts/number of blastocysts) × 100. p < 0.05 was considered statistically significant.