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List of fluorescent dyes and their concentrations used in this protocol.
| Fluorescent dyes | Assays/endpoints | Stock conc. in DMSO (mM) | Working conc. in saline solution (μM) |
|---|---|---|---|
| Hoechst 33342 | DNA damage | 10 | 10 |
| Tetramethyl rhodamine methyl ester (TMRM) | Mitochondrial impairment | 50 | 0.5 |
| Monochlorobimane (mBCl) | Intracellular glutathione level/oxidative stress | 200 | 200 |
| Calcein AM | Cell membrane integrity/cell viability | 1 | 1 |
| YO-PRO-1 | Apoptosis | 1 | 5 |
