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. 2021 Apr 12;9:657623. doi: 10.3389/fcell.2021.657623

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Copyright © 2021 Chen, Ma, Deng, Lin, Gao, He, Lu, Pi, He, Zhou, Zhang, Yu and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Neural stem cells (NSCs) identification and differentiation. (A) Cells isolated from E11.5 mouse telencephalons. Scale bar, 100 μm. (B) The single cells formed neurospheres after 6 days of in vitro culture. Scale bar, 100 μm. (C) SOX2 and NESTIN staining of cultured neurospheres. Scale bar, 50 μm. (D) EdU staining of cultured neurospheres. Scale bar, 50 μm. (E) Statistic data of the percentage of SOX2⁺, NESTIN⁺, and EdU⁺ cells. (F–H) TUBB3 and GFAP staining and statistic data of NSC-derived cells. Scale bar, 50 μm.