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Primer sequences for real-time-PCR
| Genes | Primer sequences (5′-3′) | Product length, bp |
|---|---|---|
| GAPDH | F: GATGGTGAAGGTCGGAGTGAAC | 153 |
| R: TGGGTGGAATCATACTGGAACA | ||
| Bax | F: CCGAAATGTTTGCTGACG | 232 |
| R: AGCCGATCTCGAAGGAAGT | ||
| Bcl-2 | F:ACCTGAATGACCACCTAGAGC | 281 |
| R:TCCGACTGAAGAGCGAAC | ||
| Caspase-3 | F:TTGGACTGTGGGATTGAGACG | 165 |
| R:CGCTGCACAAAGTGACTGGA | ||
| Cyto C | F:CTGCGAGTGGTGGATTGT | 222 |
| R:ATGCCTTTGTTCTTGTTGG | ||
| PGC-1α | F: TTCCGTATCACCACCCAAAT | 137 |
| R: ATCTACTGCCTGGGGACCTT |
