Figure 2 -. The effect of miR-34a on contractile proteins. (A) Real-time PCR was used to test SM-MHC and SM22 mRNA level when transfected with miR-34a mimics and inhibitor in smooth muscle cells, *p < 0.05, **p < 0.01, compared to miRNA NC group. (B) Western blot was used to test SM-MHC and SM22 protein level when transfected with miR-34a mimics and inhibitor in smooth muscle cells, quantification was calculated based on light density of bands in Quantity One, **p < 0.01 compared to miRNA NC group. (C) Real-time PCR was used to test MMP-2, MMP-3 and MMP-9 mRNA level when transfected with miR-34a mimics and inhibitor in smooth muscle cells, **p < 0.01 compared to miRNA NC group. (D) Western blot was used to test SM-MHC and SM22 protein level when transfected with miR-34a mimics and inhibitor in smooth muscle cells, quantification was calculated based on light density of bands in Quantity One, **p < 0.01 compared to miRNA NC group. (E) Transwell assay was used to detect migration ability of smooth muscle cells when treated with miR-34a mimcs/inhibitor, quantification was calculated based on OD value in each group and normalized to miRNA NC group, *p < 0.05, **p < 0.01 compared to miRNA NC group.