Figure 4 -. Target confirmation between CXCR3 and miR-34a. (A) The software miRmap was used to predict the target between CXCR3 and miR-34a. (B) Luciferase assay was used to identify direct target between CXCR3 and miR-34a, the target sequence was mutated and cloned into luciferase reporter plasmid, the luciferase activity decreased in CXCR3 WT group, **p < 0.01 compared to CXCR3 MUT group. (C) Western blot was used to detect CXCR3 protein level with miR-34a mimics transfection, quantification was calculated based on light density of bands, miR34a mimics reduced protein level compared to miRNA NC, **p < 0.01. (D) Real-time PCR was used to test CXCR3 with miR-34a mimics transfection, miR-34a inhibited mRNA expression of CXCR3 compared to miRNA NC treatment, **p < 0.01.