Figure 5 -. The effect of CXCR3 on cell proliferation and contractile proteins expression. (A) CCK-8 kit was used to detect proliferation of VSMCs when transfected with CXCR3 OV and empty vector, CXCR3 overexpression induced proliferation compared to empty vector group, **p < 0.01. (B) Transwell assay was used to detect migration ability of smooth muscle cells when treated with CXCR3 OV and empty vector, quantification was calculated based on OD value in each group and normalized to empty vector group, *p < 0.05, **p < 0.01. (C) Western blot was used to test SM-MHC, SM22, MMP-2, MMP-3 and MMP-9 protein level when transfected with CXCR3 OV and empty vector in smooth muscle cells. (D) CCK-8 kit was used to detect proliferation of VSMCs when transfected with CXCR3 OV and empty vector or co-transfected with miR-34a mimics, co-transfection of miR-34a and CXCR3 OV reversed the proliferation rate of VSMCs compared to CXCR3 OV group, **p < 0.01. (E) Transwell assay was used to detect migration ability of smooth muscle cells when treated with CXCR3 OV and empty vector or co-transfected with miR-34a, quantification was calculated based on OD value in each group and normalized to empty vector group, co-transfection of miR-34a and CXCR3 OV reversed the migration rate of VSMCs compared to CXCR3 OV group **p < 0.01. (F) Western blot was used to test SM-MHC, SM22, MMP-2, MMP-3 protein level when transfected with CXCR3 OV and empty vector or co-transfected with miR-34a mimics in smooth muscle cells, quantification was calculated based on light density of bands in Quantity One, **p < 0.01.