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. 2021 Mar 4;17(3):e1009370. doi: 10.1371/journal.ppat.1009370

Fig 6. Challenge rice Osdja5 knock-out mutants with RSV.

Fig 6

(A) Diagram of frameshift mutations in Osdja5-1 and Osdja5-2. The blue bases represent the PAM sequence, and red bases mark mutation sites. (B) The phenotype of Osdja5 knock-out mutants. (C and D) The symptom (C) and virus accumulation levels (D) of Osdja5 mutants and wild type plants infected by RSV at 12 dpi. The virus accumulation levels were detected by western blotting using a monoclonal antibody against RSV CP and Actin was used as a loading control here. (E) Analysis of the effects of OsDjA5 to the NSvc4 rescued cell-to-cell movement of PVX(Δp25)-eGFP. Agrobacterium carrying OsDjA5- FLAG or empty vector was mixed with agrobacteria carrying 800-fold-diluted PVX(Δp25)-eGFP, NSvc4, and TBSV p19. Each mixture was infiltrated into each half of the same leaf. The leaves were observed under fluorescence stereoscopic microscopy at 5 dpi. The area of eGFP fluorescence was measured by ZEN lite software and analyzed by student’s t-test (two-sided, **P < 0.01); the mean and the number of measured area (n) were labeled. Western blotting confirmed the expression of OsDjA5-FLAG.