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. 2021 Apr 12;14:576038. doi: 10.3389/fnmol.2021.576038

FIGURE 2.

FIGURE 2

N-AβCore normalizes AMPAR1 expression in an in vitro Aβ neurotoxicity model of primary hippocampal neurons and in vivo in hippocampi of 5xFAD APP/PS1 mice. (A) Normalized average expression of AMPA receptor GluA1 and GluA2 transcripts in B6.SJL mouse hippocampal neuron cultures exposed to full-length Aβ1–42 (Aβ42), the N-Aβcore (Aβ10–15) or the combination (combo), as measured via qPCR. Data are means ± SD. N = 3 cultures for each condition. ***p = 0.000001 (Bonferroni post hoc tests for one-way ANOVA comparison between Aβ42 and Aβ10–15). There was no statistical difference between expression for any condition for GluA2. (B) Expression of pAMPAR1 (pGluA1) or total AMPAR1 (GluA1) in the hippocampi of 5xFAD mice injected with sterile saline or N-Aβcore (Core), as measured using western immunoblot. N = 4 mice for each condition. Total GluA1: *p < 0.0001 (Chi-square).