FIGURE 5.
Impact of N-Aβcore on regulation of various PI3 kinase, Akt and mTOR transcripts by Aβ. Primary mouse hippocampal neuron cultures were treated with 1 μM full-length Aβ42, 1 μM Aβ10–15 (N-Aβcore) or the combination (combo). Extracted RNA was subjected to qPCR, probing for Class 1 PI3 kinase catalytic subunits (CA and CB), Akt1 and Akt2 and mTOR. Data are expressed as averaged fold-change over untreated controls, after normalizing for GAPDH expression. Bars are means ± SD. *p = 0.00063; **p = 0.000002; ***p = 0.0001; #p = 0.000001 (Bonferroni post hoc tests for one-way ANOVA comparisons, as shown). For each condition, N = 3 cultures.