Table 1. Characteristics of seven infected or colonized patients and of each E. coli strain harboring mcr-1 from three Brazilian hospitals, between 2016-2018.

Patient	1	2	3	4	5	6	7
Gender	Female	Female	Male	Female	Female	Female	Male
Age (in years)	55	87	57	82	77	91	3
Hospital	A	B	C	B	B	B	B
Distance from Hospital A	NA	6.2 km	2.7 km	6.2 km	6.2 km	6.2 km	6.2 km
Unit	Emergency Room	Emergency Room	Nursery	Emergency Room	Emergency Room	Emergency Room	Pediatric intensive care
Date of Isolation	March 2016	November 2016	August 2016	April 2017	May 2017	December 2017	January 2018
Source	Feces	Urine	Postoperative Wound Secretion	Urine	Urine	Urine	Urine
Underlying Diseases	Hepatic encephalopathy, diabetes	Urinary tract infection	Myocardial revascularization (postoperative wound infection), diabetes	Atrioventricular block, urinary tract infection	Urinary tract infection	Alzheimer, chronic renal failure, breast cancer	Neurologic disease
Rural Area Exposure	Yes	Unknown	No	Unknown	Unknown	Unknown	No
E. coli ST	ST744	ST131	ST10	ST69	ST48	ST354	ST57
GenBank accession number	JAATKQ000000000	VHOI00000000	JAATKV000000000	JAATKU000000000	JAATKT000000000	JAATKS000000000	JAATKR000000000
COL MIC	>4 µg/mL	4 µg/mL	>4 µg/mL	>4 µg/mL	>4 µg/mL	>4 µg/mL	>4 mg/mL
MER MIC	<1 µg/mL	<1µg/mL	<1 µg/mL	<1 µg/mL	<1 µg/mL	<1 µg/mL	<1 µg/mL
MIC of other antimicrobials (µg/mL) (Resistance)	DOX (>16), GEN (>8), TOB (>8), SXT (>4/76), LEVO (>8), TAZ (16), FOT (8), A/S (32/16), TIM (128/2)	DOX (16), GEN (>8), TOB (>8), LEVO (>8)	DOX (16), SXT (>4/76), AZT (>16), FEP (>16), FOT (>32), A/S2 (>64/32), P/T (>64/4), TIM (>128/2)	SXT (>4/76), A/S (32/16)	TOB (>8), FEP (16), FOT (32), A/S (64/32)	DOX (16), SXT (>4/76), LEVO (>8), FOT (32), A/S (32/16)	GEN (>8), SXT (>4/76), LEVO (>8), AZT (16), FOT (>32), A/S (32/16)
Resistance Genes	aadA1; aac(3)-VIa; aph(3’)-Ia; aac(6’)Ib-cr; aadA5; aph(6)-Id; aph(3”)-Ib; blaTEM-1B; blaOXA-1; blaCMY-2. mcr-1; aac(6')-Ib-cr. MLS: mph(A); catB4; catA1; sul2; sul1; tet(B); dfrA14; dfrA17	aac(3)-IId; aph(6)-Id; blaTEM-1B; mph(A); sul2; tet(A); mcr-1 *	aph(3’)-Ia; aadA2; blaCTX-M-8; blaTEM-1B; mcr-1; Inu(F); floR; qnrS1; sul3; tetA; drfA12	aadA5; blaTEM-1B; mcr-1; mph(A); sul1; sul2; dfrA17	aadB; aadA1; blaCTX-M-2; mcr-1; sul1	aadA1; aadB; strB; strA; blaTEM-1B; mcr-1; mph(B); sul1; sul2; tet(A); dfrA1	aadB; aadA1; blaCTX-M-2; mcr-1; sul1; sul2; dfrA1
Plasmids detected (replicon linked to mcr-1)	IncI1; IncQ1; IncX4; IncFIB(AP001918); IncFIC(FII)	IncFII(pRSB107); IncFIA; Col(BS512); IncFII(pHN7A8); IncFIB(AP001918); IncX1; (not determined)	IncX4; Incl1; IncFIA; IncFIB(AP001918); IncX1; IncFII(pRSB107); IncR	IncFIB(AP001918); IncFII(29); IncX4; Col156	ColRNAI; Col(MG828); IncA/C2; IncFIC(FII); Incl1; IncI2; IncFIB(AP001918).	p0111; IncQ1; IncX4; IncFII; ColRNAI	IncI2; IncFIB(AP001918); IncFIC(FII); Col(MG828)
mcr-1 detected in transconjugant	Yes	No	Yes	Yes	No	Yes	Yes
Virulence Factors	iroN; tsh; cma; iss; gad	gad; sat; iha; iss	iss	sat; iha; gad; eilA; iss; lpfA; senB	iss; iroN; mchF; tsh; gad	mcmA; mchF; lpfA; eilA; air; cma; gad	Iss; iroN; tsh; cma; gad

NA = not applicable; COL = colistin; MER = meropenem; DOX = doxycycline; GEN = gentamicin; TOB = tobramycin; SXT = trimethoprim-sulfamethoxazole; LEVO = levofloxacin; TAZ = ceftazidime; FOT = cefotaxime; A/S = ampicilin-sulbactam; TIM = ticarcilin-clavulanate; FEP = cefepime; AZT = aztreonam; P/T = piperacilin-tazobactam. iroN (enterobactin siderophore receptor protein); tsh (temperature-sensitive hemagglutinin); cma (colicin M); iss (increased serum survival); gad (glutamate decarboxylase); sat (secreted autotransporter toxin); iha (adherence protein); eilA (salmonella HilA homolog); lpfA (long polar fimbriae); senB (plasmid-encoded enterotoxin); mchF (ABC transporter proein MchF); mcmA (microcin M part of colicin H); air (enteroaggregative immunoglobulin repeat protein).

^*-mcr-1 gene in patient 2 was detected only by PCR and Sanger's sequencing (not by Whole Genome Sequencing).