Fig. 2.

Effect of mitochondrial antioxidants on mitochondrial ROS levels in embryos. Cleaved embryos (> 4-cell stage at 2 days after parthenogenetic activation) were cultured in medium containing vehicle (ethanol 1:1000, Con) or mitochondrial antioxidants (0.1 µM MitoT or 0.5 µM MitoQ) for 24 h. Relative fluorescence intensities of embryos stained with MitoSOX were compared among the three groups. The fluorescence intensity of the control was normalized to 1.0. a–b, P < 0.05. Number of embryos (n) examined is stated at the bottom of the figure.