Fig. 5.

Effect of mitochondrial antioxidants on expression levels of NRF2 in the nucleus. Cleaved embryos (> 4-cell stage embryos at 2 days after parthenogenetic activation) were cultured in medium containing (ethanol 1:1000, Con) or mitochondrial antioxidants (0.1 µM MitoT or 0.5 µM MitoQ) for 3 days. A: Representative images of embryos stained to visualize NRF2 and nuclei (DAPI) to examine the nuclear translocation of NRF2. B: Relative fluorescence intensities of NRF2 in the nucleus were compared among the three groups. The fluorescence intensity of control embryos was normalized to 1.0. a–b, P < 0.05. Number of embryos (n) examined is stated at the bottom of the figure.