Table 2. Effect of mitochondrial antioxidants on developmental competence of porcine embryos.
Group | No. of | No. of | No. of | Rate of | n= | TCN |
---|---|---|---|---|---|---|
replicates | embryos | blastocysts | blastocyst (%) | |||
Control | 25 | 522 | 147 | 28.5 ± 3.2 | 89 | 37.8 ± 1.1 |
MitoT | 25 | 526 | 158 | 30.5 ± 3.2 | 105 | 37.5 ± 1.0 |
MitoQ | 25 | 534 | 171 | 32.2 ± 3.2 | 113 | 35.0 ± 1.3 |
At day 2 post-activation, > 4-cell stage embryos were cultured in medium containing ethanol (Con, 1:1000), and antioxidants (0.1 µM MitoT or 0.5 µM MitoQ) for 5 days, and the rate of blastocyst per embryos were examined. Total cell number (TCN) of the blastocysts and number of blastocyst (n) examined are shown. Data are presented as the mean ± SEM.