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. 2021 Mar 26;14(3):605–614. doi: 10.1038/s41385-021-00382-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Representative flow cytometric plots of CD69 versus CD103 on CD8⁺ Tn (CCR7⁺CD45RA⁺; orange; gating control) and CD8⁺ Tem (CCR7^-; black; %) cells in fetal (F; blue), infant (I; purple), and adult (A; gray) intestinal tissues. b Frequencies (%) of CD69⁺CD103⁺ CD8⁺ Tem cells. c Frequencies (%) of CD27⁺ and CD28⁺ cells within CD69⁺CD103⁺ CD8⁺ Tem cells. d Frequencies (%) of CD69⁺CD103⁻ CD8⁺ Tem cells. Error bars represent median percentage ± IQR. The figure represents intestinal epithelium (fetal n = 7; infant n = 9; adult n = 5) and lamina propria (fetal n = 9, infant n = 9, adult n = 10) tissues. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, all Mann–Whitney U analyses.