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. 2021 Mar 5;14(3):667–678. doi: 10.1038/s41385-021-00375-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Human CELA2A cDNA was amplified from Caco2 transcripts after RT-PCR experiment. b ELA2A immunostaining on polarized Caco-2 cells cultured on transwells. Orthogonal view of Caco-2 cell monolayer showing ELA2A staining in green and ACTIN network in blue and nuclei in grey. This picture is representative of three independent experiments. Scale bar = 10 µm. c Human CELA2A transcript was amplified from isolated colonic crypt from healthy biopsy after RT-PCR experiment. ELA2A immunostaining on longitudinal section (d) and on Z cross-section (e) of healthy area from human colonic resection. Pictures are representative images of more than nine slices acquired from each individual. Signal of ELA2A is in green and signal related to epithelial cell marker, cytokeratin 18 (CK18), is in red. Nuclei are detected by DAPI staining and appear in blue. d Scale bar = 50 μm. e Scale bar = 25 μm.