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. 2020 Dec 10;14(3):640–651. doi: 10.1038/s41385-020-00358-3

Fig. 1. Oral immunotherapy (OIT) desensitizes mast cells in local intestinal mucosa.

Fig. 1

a BALB/c mice showing allergic diarrhea were treated by OIT protocols or left untreated and the status of their stools was evaluated (Allergy, n = 15; OIT n = 22). b The water content of the stools was measured (Unsensitized, n = 3; Allergy, n = 6; OIT, n = 4), **P < 0.01, *** P < 0.001. N.S. indicates not significant. c Percentages of colonic MCs were examined by flow cytometry. Cells were gated on CD45+ c-kit+ FcεRIα+ cells. Percentages of MCs among CD45+ cells are shown (Non-allergic, n = 3; Allergy, n = 10; OIT, n = 8). *P < 0.05, ** P < 0.01. d Serum mouse mast cell protease-1 (mMCPT1) concentrations were measured by ELISA. (Allergy, n = 5; OIT, n = 4). e CD63 expression on colonic MCs was examined by flow cytometry. Cells were gated on c-kit+ and FcεRIα+ cells and the percentages of CD63+ MCs among all MCs were determined by flow cytometry. Data are representative of nine mice. f CD63+ MCs among CD45+ cells are shown (Intact, n = 3; Allergy, n = 9; OIT, n = 9). *P < 0.05, ** P < 0.01. g IL-4 cytokine expression was analyzed by quantitative RT-PCR. Each result was normalized against the expression of Gapdh. Data are shown as means ± SEM, *P < 0.05. N.S., not significant.