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. 2020 Dec 10;14(3):640–651. doi: 10.1038/s41385-020-00358-3

Fig. 4. Involvement of mast cells in expansion of Foxp3 Treg populations upon oral immunotherapy (OIT).

Fig. 4

a Tim4 and CD11b and (b) CD11b and CD103 were both gated for CD11c+ CD45+ and examined by flow cytometry. The numbers shown indicate the percentages of cell populations. Data represent three individual experiments. c Protocol used in this experiment. Black bars indicate the doses of heated ovalbumin (OVA), and the red triangle indicates the administration of 25 mg of unheated OVA. Diphtheria toxin (DT) was administered to Mas-TRECK mice during OIT. d and e OIT mice (wild-type [WT] or Mas-TRECK transgenic [tg] mice) received DT from days 5 through 36, and Foxp3-expressing Tregs were observed by flow cytometry on day 37 upon inoculation of unheated 25 mg of OVA. Data are gated for CD45 and representative of at least three individual experiments. Data are shown as means ± SEM, **P < 0.01. f CD25+ CD39+ CD103+ CD4+ T cells were isolated, and IL-10 and TGF-β gene expression was analyzed by quantitative RT-PCR. Data are shown as means ± SEM, *P < 0.05 (all groups, n = 4).