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. 2020 Dec 10;14(3):640–651. doi: 10.1038/s41385-020-00358-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a DNP-IgE bound (None), antigen-stimulated (Activation), or desensitized (Desensitization) bone marrow MCs (BMMCs) were co-cultured with CD4⁺ T cells and expansion of the Foxp3⁺ CD4⁺ T cell population was analyzed by flow cytometry and cell sorting. Numbers indicate percentages of Foxp3⁺ among all CD4⁺ T cells. b IgE-bound and desensitized BMMCs and CD4⁺ T cells were co-cultured under separated conditions in transwells. Percentages of Foxp3⁺ cells among all CD4⁺ T cells are shown. c Gene microarray was performed with DNP-IgE bound (None), antigen-stimulated (Activated), and desensitized BMMCs. Shown is a heatmap representative of entities termed cytokines. d Expression of the genes encoding IL-2 and IL-6 was analyzed by quantitative RT-PCR. Data are shown as means ± SEM. Act., activated; Des., desensitized. e IL-2 production was analyzed by ELISA. Data are shown as means ± SEM. ***P < 0.001 (all groups, n = 5). f IL-2 production from hPBMC was analyzed by ELISA. Data are shown as means ± SEM; all groups, n = 3. g Percentages of Foxp3⁺ cells in coculture of desensitized BMMCs are shown. Isotype or anti-IL-2 antibody was added. h Gene expression of in vivo sorted MCs was analyzed by quantitative RT-PCR. Each result was normalized against the expression of Gapdh. Data are shown as means ± SEM, ***P < 0.01, *P < 0.05.