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. 2021 Mar 5;14(3):691–702. doi: 10.1038/s41385-021-00380-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A Representative TEM micrographs of Ple^fl/fl and Ple^ΔIEC IEC junctional complexes. Braces (white) indicate hemidesmosomes (HD), tight junctions (TJ), adherens junctions (AJ), and desmosomes (Ds). Scale bar, 500 nm. Graphs show quantitative analyses of junctional complex widths (measured as the distance from IEC to BM (HD) or distance from IEC to IEC membrane (TJ, AJ, and Ds)). Five to fifteen junctions were measured (two mice per genotype). B Relative mRNA levels of integrin (Itg) α6 and β4 in scraped mucosa from Ple^fl/fl and Ple^ΔIEC distal colons, n = 4–5. C Quantification of Itgβ4 and Itgα6 in scraped distal colon mucosa from Ple^fl/fl and Ple^ΔIEC mice by immunoblotting. GAPDH, loading control. The graph shows relative band intensities normalized to average Ple^fl/fl values, n = 3. D Relative mRNA levels of ZO-1, E-cadherin (E-cad), desmoglein 2 (Dsg2), and desmoplakin 1/2 (Dsp1/2) in Ple^fl/fl and Ple^ΔIEC distal colons, n = 5. E Quantification of ZO-1, E-cad, and Dsg2 in Ple^fl/fl and Ple^ΔIEC colon mucosa by immunoblotting. GAPDH, loading control. The graph shows relative band intensities normalized to average Ple^fl/fl values, n = 3. Data are presented as mean ± SEM, n.s. not significant, *P < 0.05, **P < 0.01, ^†P < 0.001.