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. Author manuscript; available in PMC: 2022 May 1.
Published in final edited form as: Kidney Int. 2021 Jan 4;99(5):1102–1117. doi: 10.1016/j.kint.2020.12.022

Figure 2: Glomerular and podocyte Myo9A expression.

Figure 2:

A) Immunoblots show Myo9A in mouse kidney, immortalized mouse podocytes (1), undifferentiated (2) and differentiated (3) human podocytes; B) Immuno-fluorescence microscopy (IF) shows MYO9A in the cytoplasm of undifferentiated and differentiated human podocytes (green), partially co-localized with F-actin (red) in differentiated podocytes (yellow), scale bar=10μm; C) IF shows Myo9A (red) and podocin (green) partially co-localized in mouse glomeruli (merge=yellow), scale bars=50μm; D) Dual immunolabeling and confocal microscopy show Myo9A staining (red) on podocytes tightly contiguous to podocin (green) on its GBM side (arrowheads), insets show higher magnification; scale bar = 25μm. E) Confocal microscopy shows Myo9A staining closely contiguous or co-localized with laminin (green) along the outer aspect of the GBM (insets and arrowhead); scale bar = 25μm. F an G) Immuno-EM localization of Myo9A in glomeruli of wild type mouse kidneys: gold particles (black arrows) are observed in the podocyte cell body (pod) and the foot processes cytoplasm (fp), some gold particles are seen at the fp base next to the GBM but not at slit diaphragms; scale bars=500 nm.