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. Author manuscript; available in PMC: 2022 Apr 1.
Published in final edited form as: Neurobiol Learn Mem. 2021 Feb 18;180:107404. doi: 10.1016/j.nlm.2021.107404

Figure 4. Females have increased baseline promoter 5-hydroxymethylation and expression of the ubiquitin coding gene Uba52 in the amygdala.

Figure 4.

(A) Females have increased baseline levels of free ubiquitin in the basolateral amygdala (BLA; n = 8-10 per group) (B). qRT-PCR analysis of RNA collected from BLA of naïve male and female rats (n = 7-8 per group). Females had elevated baseline levels of Uba52, but not Ubb, Ubc or Rps27a, in the BLA. (C) Schematic of the Uba52 gene showing CpG island and site of methylated DNA immunoprecipitation (meDIP) PCR analysis. (D-E) DNA 5-hydroxymethylation was increased at the Uba52 promoter (D) but not coding (E) region in females (n = 7 per group). (F) Bisulfite sequencing of 5 CpG sites in the Uba52 promoter in naïve males and females revealed an increase in methylation at CpG4 in females (n = 5-7 per group). * p < 0.05 from Males.