Fig. 4.

Specific expression of GPR17 and P2RX7 in mouse oligodendroglial cells. Transcript signals values of mouse brain cells from publically available datasets of bulk sequencing [80] and single-cell sequencing [40] were analysed using the DeSeq2, Seurat and ggplot2 packages in RStudio, following standard published procedures [59, 63]. Bulk sequencing profiles indicate highest expression of P2rx7 corresponds to Gpr17 in OPCs and COPs, compared to other cells. This was confirmed in single-cell sequencing profiles, which indicate a marked decrease in expression levels of both P2rx7 and Gpr17 in aged OPCs (22 months of age, 22 M) compared to adult OPCs (2 months of age, 2 M)