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. 2021 Apr 12;9:640402. doi: 10.3389/fcell.2021.640402

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Copyright © 2021 Li, Tian and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of circGFRα1 in FGSCs. (A) The genomic locus of circGFRα1. (B) RT-PCR products showing circularization of circGFRα1 with divergent primers. (C) After RNase R treatment in FGSCs, qRT–PCR showing the expression of circGFRα1 and GFRα1 mRNA. (D) Sanger sequencing of circGFRα1 demonstrating the head-to-tail splicing. (E) After Actinomycin D treatment, qRT–PCR showing the expression of circGFRα1 and GFRα1 mRNAs at the indicated time points. (F) RNA FISH for circGFRα1. Scale bars, 20 μm. ***P < 0.001.