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. 2021 Apr 12;9:640402. doi: 10.3389/fcell.2021.640402

FIGURE 2.

FIGURE 2

CircGFRα1 affects self-renewal and survival of FGSCs. (A) Selected images for FGSCs infected with lentivirus. (B) qRT-PCR analyses detected the RNA level of circGFRα1 in cells infected with the circGFRα1 overexpression lentivirus control (over-con), circGFRα1 overexpression lentivirus (over). (C) qRT-PCR analyses detected the RNA level of circGFRα1 in cells infected with the circGFRα1 knockdown lentivirus control (kd-con), circGFRα1 knockdown lentivirus (kd). (D) CCK-8 assays were conducted using FGSCs infected with the circGFRα1 overexpression lentivirus control (over-con), circGFRα1 overexpression lentivirus (over), circGFRα1 knockdown lentivirus control (kd-con), circGFRα1 knockdown lentivirus (kd). (E) EDU assays were conducted using FGSCs infected with the circGFRα1 overexpression lentivirus control (over-con), circGFRα1 overexpression lentivirus (over), circGFRα1 knockdown lentivirus control (kd-con), circGFRα1 knockdown lentivirus (kd). (F) Relative expression of Akt, Bcl6b, Lhx, and Etv5 in FGSCs after circGFRα1 overexpression (over) and knockdown (kd). Overexpression control and knockdown control as blank control groups respectively. When calculating the relative expression, the expression of control group was set as 1. A positive value indicates up regulation, and a negative value indicates down regulation. (G) Relative expression of Stra8, Sycp3, and Kit, FGSCs infected with the circGFRα1 knockdown lentivirus control (kd-con), circGFRα1 knockdown lentivirus (kd). **P < 0.01 and ***P < 0.001.