FIGURE 5.

METTL14 promotes cytoplasmic export of m⁶A methylated circGFRα1. (A) MeRIP assay showing that m⁶A was highly enriched in circGFRα1 (B) Relative m⁶A level of FGSCs after METTL14 knockdown (kd). (C) MeRIP assay showing that down- regulation of METTL14 resulted in the decreased m⁶A level of circGFRα1. (D) RNA-FISH showing that the increased nuclear staining of circGFRα1 caused by METTL14 knockdown. (E) Cytoplasmic and Nuclear RNA Fractionation assay showing that knockdown of METTL14 increased the nuclear circGFRα1 content. (F,G) Cytoplasmic and Nuclear RNA Fractionation assay showing that the nuclear and cytoplasmic circGFRα1 contents were both increased, and mainly in nuclear fraction when mutated the GGACU m⁶A motif in circGFRα1 overexpressing construct. Scale bars, 20 μm. ***P < 0.001.