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. 2021 Apr 12;9:665919. doi: 10.3389/fcell.2021.665919

FIGURE 5.

FIGURE 5

CAV-1 is required for the cell polarized morphology establishment and directional cell migration. (A) Phase contrast imaging reveals the morphology of WT and CAV-1 KO cells. Bars, 50 μm. (B) The elliptical factor (length/breadth) were calculated for WT (n = 63) and CAV-1 KO (n = 63) cells. (C) Representative images form time-lapse videos of WT and CAV-1 KO cells migrating in 50 μm width and 50 μm height channels with fibronectin-coated surface. Color lines indicate the trajectory of each single cell nucleus stained by Hoechst and the kymograph are shown in the lower panel. Bars, 50 μm. (D,E) Migration velocities (D) and directionalities (E) of WT and CAV-1 KO cells. Quantification is based on tracking of the displacement of nuclei. The data are presented as average velocity obtained from a 10 h cell tracking. n = 21 cells for each group in panel (D) and 20 cells in panel E. In (B,D), ***P < 0.001 (Mann-Whitney test); In (E), ***P < 0.001 (unpaired t-test). All the data are from three independent experiments. (F) A working model illustrates the negative feedback loop between actin assemblies and cytoplasmic CAV-1.