Figure 2.

Determination of rhodopsin lifetime and phototransduction amplification constant by fitting the flash response model to the response leading edge (Eq. (15)) to the activation phase of the population-averaged LERG-OS response family recorded in low Ca²⁺ solution with twofold increments in flash strengths. (a) The outcome of fitting the model with different τ_R values to the response family. The black curve shows the optimal amplification constants leading to the best fit with different τ_R values, and the red curve shows the sum of squared errors of the fits. The combination of τ_R and A values leading to the overall best fit (lowest sum of squared error, the minimum of the red curve) of the model was τ_R = 26 ms and A = 21.8 s⁻². (b) A population-averaged response family in low Ca²⁺ (n = 13) and the model fittings using τ_R = 26 ms and A = 21.8 s⁻². Response families were recorded with flash strengths ranging from 1.4 to 177 R*rod⁻¹. The model was fitted to all responses up to the first saturated response.