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. 2021 Apr 26;11:8988. doi: 10.1038/s41598-021-88532-w

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Change in number of mismatches between two occurrences over delta time between the two occurrences for the IP4 primer developed in France. The increasing slope shows that mutations are being sustained as we compare genomes that occur further apart in time. Graphs for all primers are included in the supplement.