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. 2021 Apr 26;11:8977. doi: 10.1038/s41598-021-88413-2

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Immune protection efficacy of EBY/pYD1-HA against different H5N1 viruses. Chickens were intranasally challenged with a lethal dose (25 μL of 10⁴ EID₅₀) of H5N1 virus. (a) Weight change as a percentage after A/Vietnam/1203/2004 (H5N1) (clade 1) challenge. (b) Weight change as a percentage after A/Chicken/Henan/12/2004 (H5N1) (clade 8) challenge. (c) Lung viral titers at day 3 post A/Vietnam/1203/2004 (H5N1) (clade 1) challenge. (d) Lung viral titers at day 3 after A/Chicken/Henan/12/2004(H5N1) (clade 8) challenge. (n = 3 chickens per group). (e) Survival rates after A/Vietnam/1203/2004 (H5N1) (clade 1) challenge. (f) Survival rates after A/Chicken/Henan/12/2004(H5N1) (clade 8) challenge. (n = 10 per group). The weight change data are presented as the means ± SDs. Asterisks indicate significant difference compared with the PBS- and EBY100/pYD1 controls. (*p ˂ 0.05).