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. 2021 Apr 26;11:8963. doi: 10.1038/s41598-021-88355-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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IHC and western blot assays of MDA-MB-231 and MDA-MB-468 xenograft tumors in nude mice. (A) Representive photographs of CSE staining in MDA-MB-231 and MDA-MB-468 xenograft tumors; original magnification ×200 and ×400. (B, C) CSE-positive expression level was calculated as areal density = IOD/AREA, where IOD represents positive cumulative optical density values and AREA represents the pixel area of the organization. Values are presented as mean ± SEM (n = 5); *P < 0.05 compared with the control group; ^△P < 0.05 compared with PAG group. (D, E) CSE and VEGF expression analysis in MDA-MB-231 and MDA-MB-468 xenograft tumors. (F, G) The statistical analysis of CSE and VEGF expression level in MDA-MB-231 and MDA-MB-468 xenograft tumors; Values are presented as mean ± SEM (n = 5); *P < 0.05 compared with the control group; ^▲P < 0.05 compared with PAG group.