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. 2021 Apr 26;11:8921. doi: 10.1038/s41598-021-87959-5

Figure 3.

Figure 3

Introduction of point mutants to improve GFRAL binding affinity. (a) Structure of GDF15 monomer. Highlighted residues at the GFRAL binding site were selected for mutation (PDB 5VZ4). (b) GDF15 point mutations designed to improve GFRAL binding affinity. (c) Predicted engineered interactions modeled on GDF15-GFRAL crystal structure (PDB 5VZ4). Wildtype interaction shown above. Theoretical up-mutant interactions shown below. Blue, hGDF15. Yellow, hGFRAL. Yellow dashed lines denote predicted hydrogen bonding interactions. Figures rendered using The PyMOL Molecular Graphics System, Version 2.0 Schrödinger, LLC (https://pymol.org/).