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Fc-GDF15 glyco variants contain sialidase sensitive glycans. (a) Representative glycan profiling trace using capillary gel electrophoresis, comparing wildtype Fc-GDF15 control (Fc-WT, blue) and Mutant 2 (red). LM, lower marker. Asterisks denote sialidase sensitive peaks. Pound symbols denote unannotated peaks that did not align to glycan standards. (b) Summary table of major identified glycan species for Fc-GDF15 WT control and variants.
