. 2020 Aug 19;58(6):2133–2147. doi: 10.1007/s13197-020-04723-9

Table 3.

Antioxidant and antibacterial activities of ethanol fraction isolated from Russula alatoreticula as represented by EC₅₀ and MIC values (μg/ml) respectively

Parameters to detect bioactivity		Russula alatoreticula	Standard
Antioxidant activity
EC₅₀ value	Scavenging ability of DPPH radicals	785 ± 40^a	7.45 ± 0.02^b
	Scavenging ability of ABTS radicals	1300 ± 32^a	3.65 ± 0.02^b
	Chelating ability of ferrous ion	1500 ± 27^a	2.54 ± 0.52^b
	Reducing power	2500 ± 37^a	32.21 ± 6.91^b
Total antioxidant activity (μg ascorbic acid equivalent/mg of dry extract)		5.45 ± 0.12	Not applicable
Antibacterial effect
Gram positive	Listeria monocytogenes	226.5 ± 58.5^a	4.68 ± 0.17^b
	Staphylococcus aureus	88.7 ± 20.22^a	6.29 ± 0.16^b
	Bacillus subtilis	72.5 ± 23.33^a	5.61 ± 0.01^b
Gram negative	Escherichia coli	156.23 ± 25.13^a	5.41 ± 0.11^b
	Salmonella typhimurium	289 ± 15.36^a	5.09 ± 0.03^b
	Klebsiella pneumoniae	1560 ± 45.68^a	5.29 ± 0.14^b

BHA was used as a standard in all antioxidant assays except chelating ability of ferrous ion and total antioxidant capacity methods where EDTA and ascorbic acid were considered as positive controls respectively. Streptomycin was used as a reference in antibacterial methods. In each row, dissimilar letters denote significant alterations between sample and standard (p < 0.05)