Table 1.

Primers used for real-time RT-PCR

Target gene	Forward primer sequence (5′–3′)	Reverse primer sequence (5′–3′)
RUNX-2	TGGTTACTGTCATGGCGGGTA	TCTCAGATCGTTGAACCTTGCTA
Col I	TGGTGGAGCAGCAAGAGCAA	CAGTGGACAGTAGACGGAGGAAA
OCN	CACTCCTCGCCCTATTGGC	CCCTCCTGCTTGGACACAAAG
GAPDH	CTGGTAAAGTGGATATTGTTGCCAT	TGGAATCATATTGGAACATGTAAACC
ALP	GGACATGCAGTACGAGCTGA	GTCAATTCTGCCTCCTTCCA
NANOG	CAGAAGGCCTCAGCACCTAC	ATTGTTCCAGGTCTGGTTGC
OCT3/4	GTGGAGAGCAACTCCGATG	TGCAGAGCTTTGATGTCCTG
SOX2	CCAAGACGCTCATGAAGAAG	TGGTCATGGAGTTGTACTGC
GAPDH	CTGGTAAAGTGGATATTGTTGCCAT	TGGAATCATATTGGAACATGTAAACC

RUNX-2 runt-related transcription factor 2, Col I type I collagen, OCN osteocalcin, ALP alkaline phosphatase, SOX 2 SRY-related HMG-box 2