Table-2.
Biochemical and enzymatic profiles of S. algae strains from ballast water.
| Strain/Characteristics | S. algae (Sa-BW) | S. algae IAM 14159T | S. putrefaciens ATCC 8071T | S. haliotis JCM 14758T |
|---|---|---|---|---|
| Morphological characteristics | ||||
| Gram stain | − | − | − | − |
| Cell shape | rod | rod | rod | rod |
| Motility | + | + | + | + |
| Spores | − | − | − | − |
| Biochemical characteristics | ||||
| Oxidase | + | + | + | + |
| Catalase | + | NA | + | + |
| Voges–Proskauer | − | NA | NA | NA |
| Methyl red | − | NA | NA | NA |
| Indole production | − | NA | − | − |
| H2S production | + | + | + | + |
| Nitrate reduction | + | + | + | + |
| Utilization of glucose | + | + | + | − |
| Utilization of sucrose | − | − | − | − |
| Utilization of lactose | − | − | − | − |
| Utilization of maltose | − | − | − | − |
| Utilization of citrate | − | − | − | + |
| Growth on SS agar | + | + | − | NA |
| Growth on MacConkey | + | NA | NA | NA |
| Growth at 42°C | + | + | − | + |
| Growth at 4°C | − | − | + | − |
| Growth in 6% NaCl | + | + | − | + |
| Growth in 8% NaCl | + | NA | NA | + |
| Enzymatic characteristics: | ||||
| Lipase | + | + | + | − |
| Protease | + | NA | + | + |
| DNase | + | + | + | + |
| Gelatinase | + | + | NA | + |
| Hemolysis (sheep blood) | + | + | − | NA |
| Hemolysis (human blood) | + | NA | NA | NA |
T
=Type strain, NA=Not available, +=positive reaction, –=negative reaction. S. algae: Shewanella algae