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. 2021 Apr 13;12:641484. doi: 10.3389/fmicb.2021.641484

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Copyright © 2021 Mizutani, Uesaka, Ota, Calassanzio, Ratti, Suzuki, Fujimori and Chiba.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of coverage uniformity between DRS and Illumina HiSeq. Genomic coverages of each viral contig from DRS (upper graph) and Illumina HiSeq (lower graph) visualized as histograms. The Illumina contigs of F. sambucinum hypovirus 1 (FsamHV1) and F. sambucinum mitoviruses (FsamMVs) and the DRS contig polished with Illumina reads of F. sambucinum victorivirus 1 (FsamVV1) were used as reference sequences for read mapping. The x-axis indicates the nucleotide position of a viral genome, and the y-axis indicates the coverage depth at each nucleotide position.