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. 2021 Apr 13;12:650708. doi: 10.3389/fimmu.2021.650708

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Copyright © 2021 Dang, Lu, Chen and Li

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Baricitinib treatment has an immunoregulatory effect on the spleen of experimental autoimmune encephalomyelitis (EAE) mice. Splenic mononuclear cells (MNCs) were isolated for flow cytometry to analyze the percentage of CD4+IFN-γ+ Th1 (A), CD4+IL-4+ Th2 (B), CD4+IL-17+ Th17 (C), and CD4+CD25+Foxp3+ Treg cells (D) at the peak phase of EAE. The percentages of Th1 and Th17 cells in the splenic MNCs were decreased in baricitinib-treated mice compared with those in vehicle-treated mice. However, baricitinib did not affect the differentiation of CD4+IL-4+ Th2 cells and CD4+CD25+Foxp3+ Treg cells. (E–H) Quantification of the mRNA expression of T-bet, GATA, RORγt, and Foxp3 in the baricitinib-treated groups and the control group. (I) MTS proliferation assays were performed to investigate the level of splenic lymphocyte proliferation. The results are displayed as the stimulation index. Baricitinib-treated groups exhibited significant reductions in proliferation. Quantitative data are the mean ± SEM. *p < 0.05; n = 6 per group. SEM, standard error of the mean.