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. 2021 Apr 13;8:646574. doi: 10.3389/fmolb.2021.646574

TABLE 1.

Free endogenous amino acid pools in stage 5 and 6 Xenopus laevis oocytes.

GC-MS a HPLC
Amino acid EIC (m/z) Concentration (μM) pmol/oocyte (mean ± S.E.M) Range b (pmol/oocyte)
Valine 144 499 ± 121 182 ± 55 40–195
Leucine 158 77 ± 25 28 ± 9 20–44
Isoleucine 158 110 ± 36 40 ± 13 16–34
Proline 142 310 ± 110 113 ± 40 30–83
Glycine 174 64 ± 9 23 ± 3 22–92
Serine 204 290 ± 38 106 ± 14 112–263
Alanine 188 222 ± 33 81 ± 12 32–94
Threonine 218 96 ± 33 35 ± 12 16–106
Aspartic acid 232 2349 ± 1050 857 ± 383 409–2200 d
Methionine 176 124 ± 52 45 ± 19 6–27
Cysteine 220 58 ± 16 21 ± 6 9–21
Glutamic acid 246 1869 ± 669 682 ± 244 793–2000 c
Phenylalanine 216 93 ± 38 34 ± 14 10–33
Asparagine 231 650 ± 214 237 ± 78
Glutamine 156 1455 ± 104 531 ± 38
Arginine 157 288 ± 105 105 ± 38 51–188
Lysine 156 85 ± 44 31 ± 16 25–195
Histidine 154 121 ± 47 44 ± 17 28–236
Tyrosine 280 493 ± 80 180 ± 29 27–92
Tryptophan 202 33 ± 11 12 ± 4
a

Concentrations calculated for oocytes assuming an intracellular water-accessible volume of 365 nL as previously reported (Stegen et al., 2000).

b

Concentrations taken from (Taylor and Smith, 1987; Meier et al., 2002) for HPLC measurements take into account the full range of reported values included means ± S.D.

c

Includes values for both glutamate and glutamine combined.

d

Includes values for both aspartate and asparagine combined.