TABLE 1.
GC-MS a | HPLC | |||
---|---|---|---|---|
Amino acid | EIC (m/z) | Concentration (μM) | pmol/oocyte (mean ± S.E.M) | Range b (pmol/oocyte) |
Valine | 144 | 499 ± 121 | 182 ± 55 | 40–195 |
Leucine | 158 | 77 ± 25 | 28 ± 9 | 20–44 |
Isoleucine | 158 | 110 ± 36 | 40 ± 13 | 16–34 |
Proline | 142 | 310 ± 110 | 113 ± 40 | 30–83 |
Glycine | 174 | 64 ± 9 | 23 ± 3 | 22–92 |
Serine | 204 | 290 ± 38 | 106 ± 14 | 112–263 |
Alanine | 188 | 222 ± 33 | 81 ± 12 | 32–94 |
Threonine | 218 | 96 ± 33 | 35 ± 12 | 16–106 |
Aspartic acid | 232 | 2349 ± 1050 | 857 ± 383 | 409–2200 d |
Methionine | 176 | 124 ± 52 | 45 ± 19 | 6–27 |
Cysteine | 220 | 58 ± 16 | 21 ± 6 | 9–21 |
Glutamic acid | 246 | 1869 ± 669 | 682 ± 244 | 793–2000 c |
Phenylalanine | 216 | 93 ± 38 | 34 ± 14 | 10–33 |
Asparagine | 231 | 650 ± 214 | 237 ± 78 | — |
Glutamine | 156 | 1455 ± 104 | 531 ± 38 | — |
Arginine | 157 | 288 ± 105 | 105 ± 38 | 51–188 |
Lysine | 156 | 85 ± 44 | 31 ± 16 | 25–195 |
Histidine | 154 | 121 ± 47 | 44 ± 17 | 28–236 |
Tyrosine | 280 | 493 ± 80 | 180 ± 29 | 27–92 |
Tryptophan | 202 | 33 ± 11 | 12 ± 4 | — |
Concentrations calculated for oocytes assuming an intracellular water-accessible volume of 365 nL as previously reported (Stegen et al., 2000).
Concentrations taken from (Taylor and Smith, 1987; Meier et al., 2002) for HPLC measurements take into account the full range of reported values included means ± S.D.
Includes values for both glutamate and glutamine combined.
Includes values for both aspartate and asparagine combined.