Figure 6.
EPO promotes bacterial clearance and enhances ciprofloxacin actions in resolution-delayed E. coli-initiated infections. WT mice were inoculated with E. coli (5 × 106 c.f.u.) by intraperitoneal injection. After 2 hrs, mice were treated with rhEPO (5,000 IU/kg), ciprofloxacin (12.5 mg/kg), ciprofloxacin (12.5 mg/kg) plus rhEPO (5,000 IU/kg) or PBS. After 24 hrs, mice were sacrificed (n = 5). (A): Percentage and cell numbers of peritoneal PMN cells. (B): Bacterial titers of peritoneal lavage fluids. (C): Histopathology in lungs (2 mm for left bars,100 μM for right bars). The lung injury score was assessed as described in Materials and Methods. Data are representative of at least two independent experiments. Results were expressed as mean ± SEM. *P < 0.05. Statistics: one-way ANOVA with Tukey’s post hoc test for multiple comparisons (A–C).