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. 2021 Apr 13;10:596623. doi: 10.3389/fonc.2020.596623

Figure 2.

Figure 2

Knockdown of circZFR inhibits bladder cancer (BCa) cells proliferation, migration and invasion. (A) Relative circZFR levels in T24 and J82 cells after sh-circZFR#1 or sh-circZFR#2 knockdown. (**P < 0.01 vs. sh-NC group, n = 3) (B) The effects of circZFR knockdown on BCa cells viability were detected by the CCK8 assay. (**P < 0.01 vs. sh-NC group, n = 3) (C) Colony formation assay to determine BCa cells colony formation. (**P < 0.01 vs. sh-NC group, n = 4) (D, E) Transwell assay to measure BCa cells migration [(D), without matrigel] and invasion [(E), with matrigel on transwell] capacity. (magnification×100, **P < 0.01 vs. sh-NC group, n = 3) (F) Protein expression in BCa cells. (G) Changes of tumor volume in mice, which were measured per 7 days. (**P < 0.01 vs. sh-NC group, n = 5) (H) Tumor weight in two groups were measured by electronic scales. (**P < 0.01 vs. sh-NC group, n = 5).