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. 2021 Apr 13;10:596623. doi: 10.3389/fonc.2020.596623

Figure 5.

Figure 5

circZFR promotes bladder cancer (BCa) progression by regulating miR-545/1270/wnt5a and via activating Wnt/β-catenin signaling pathway. (A) Protein expression of T24 and J82 cells. (B) Overexpression of miR-1270 inhibitor, miR-545 inhibitor and WNT5A reverses the inhibitory effect of circZFR on cell proliferation revealed by CCK-8 assay (**P < 0.01 vs. sh-NC group, ##P < 0.01 vs. sh-circZFR group, n = 3) (C) Colony formation assay to determine BCa cells colony formation. (**P < 0.01 vs. sh-NC group, ##P < 0.01 vs. sh-circZFR group, n = 3) (D, E) Transwell assay to measure BCa cells migration [(D), without matrigel] and invasion [(E), with matrigel on transwell] capacity. (magnification×100, **P < 0.01 vs. sh-NC group, ##P < 0.01 vs. sh-circZFR group, n = 3) (F, G) Protein expression in T24 and J82 cells.