Figure 3.

KDM6B upregulates IRF4 through H3K27me3 modification in the promoter region in astrocytes

(A) The network diagram of KDM6B-related regulatory relationships predicted using the SIGNOR database. (B) The expression of IRF4 before and after OGD/R treatment determined by western blot analysis. (C) The mRNA expression of KDM6B and IRF4 in OGD/R-exposed astrocytes determined by qRT-PCR. (D) The protein expression of KDM6B and IRF4 in OGD/R-exposed astrocytes measured by western blot analysis. (E) The enrichment of KDM6B on genomic DNA determined by ChIP assay. (F) The level of H3K27me3 in the IRF4 promoter region after KDM6B overexpression or knockdown determined by ChIP assay. (G) The expression of inflammatory factors TNF-α, IL-1β, and IL-6 in OGD/R-exposed astrocytes determined by ELISA. Data in the figure were measurement data and expressed by mean ± standard error of mean. Comparison between two groups was analyzed by unpaired t test, and comparison among multiple groups was analyzed by one-way ANOVA with Tukey’s test. The experiment was repeated three times. ∗∗p < 0.05.