Figure 6.

Demethylase KDM6B deteriorates ischemic brain injury in mice via the IRF4/Notch2/SOX9 axis

(A) The neurological deficit score of MCAO-operated mice. (B) The behaviors of MCAO-operated mice evaluated by pole test and foot fault test. (C) The cerebral infarction area in MCAO-operated mice shown by TTC staining. (D) Astrocyte activation determined by GFAP immunofluorescence staining. (E) Neuronal apoptosis determined by TUNEL staining. (F) The expression of inflammatory factors TNF-α, IL-1β, and IL-6 in mouse serum determined by ELISA. (G) The KDM6B, IRF4, Notch2, and SOX9 mRNA expression in the brain tissue of mice determined by qRT-PCR. (H) The KDM6B, IRF4, Notch2, and SOX9 protein expression in the brain tissues of mice measured by western blot analysis. Data in the figure were measurement data and expressed by mean ± standard error of mean. Comparison between two groups was analyzed by unpaired t test, and comparison among multiple groups was analyzed by one-way ANOVA with Tukey’s test. The experiment was repeated three times. ∗∗p < 0.05.